By adding single molecule RNA detection capabilities, the RainDrop® Digital PCR System now provides the industry’s most cost-effective and deployable means of digital counting of RNA molecules. This new method for directly counting RNA molecules provides the most precise, sensitive and accurate means of analysis available.
If you are interested in looking at multiple mRNA, Viral RNA and microRNA targets expressed at both high and low levels in the same sample, be sure to watch the recent webinar featuring Michael Samuels, PhD, Principal Research Scientist and Scientific Liaison at RainDance Technologies. Dr. Samuels provides a detailed overview of the RainDrop Digital PCR System and the key advantages provided by the One-Step Single Molecule RNA Detection Capabilities.
Below are 5 Ways the RainDrop System provides unique and enabling performance for RNA analysis:
A wide dynamic range of six logs, enabling scientists to simultaneously detect very low and very high abundance targets without the need to find a ‘sweet spot’ dilution. This level of performance is crucial in many applications, including counting endogenous genes expressed at very different levels or in viral load studies where dose can vary greatly and emerging minor drug resistant strains need to be quantitatively analyzed.
Multiplex analysis of gene targets, including endogenous and exogenous controls. Uniquely, the RainDrop System allows multiplex counting of up to 10 targets in each sample, providing intrinsically normalized quantification without any calibration curves.
Extremely high precision counting, enabling detection of very subtle RNA level changes (+/- 10 percent). This will help transform understanding in many biology applications where small changes in gene expression levels can have large effects (e.g. neurobiology, stem cell research, etc.).
A flexible open access system, with standard qPCR reagents and many One-Step reverse transcription kits demonstrated to work without additional optimization.
Reverse transcription is highly efficient in this novel single-molecule format. Even direct multiplex miRNA counting works in a One-Step protocol using off-the-shelf reagents!
If you are interested in learning more about our products, please contact us by filling out this form. A RainDance representative will follow up with you to answer your questions and discuss how our technology can help advance your research.
The RainDrop Digital PCR System is for Research Use Only; not for use in diagnostic procedures.