The RainDrop® Digital PCR System is a highly sensitive tool for precise quantification of nucleic acids using standard probe-based qPCR reagents. The system features an unprecedented multiplexing capability made possible by RainDance’s proprietary digital droplet technology platform. Since each droplet encapsulates a single molecule, researchers can quickly determine the absolute number of droplets containing specific target DNA and compare that to the number of droplets with background wild-type DNA. The RainDrop System also shifts the current dPCR paradigm from a single-color-per-marker approach to a two color with varying probe intensity method that is capable of multiplexing up to 10 markers. This multiplexing capability enables more precise detection, identification, and measurement of multiple possible mutations from a single DNA sample.
In this webinar, we walked through the basic steps required to multiplex two FAM labelled probes and two TET labelled probes into a single reaction for simultaneous analysis of four different exons of the Dystrophin gene. The workflow is simple and easy and can be transferred to any combination of assays for a range of research applications, including low-frequency tumor allele detection, genotyping, gene expression, copy number variation, viral quantitation, and direct RNA detection.
About the Speaker
Adam S. Corner, Ph.D., is a Senior Field Applications Scientist at RainDance Technologies. Prior to RainDance Technologies, he held similar senior scientist and field applications positions at Ingenuity Systems and Applied Biosystems. Adam holds a B.Sc. degree in Biochemistry & Genetics and a Ph.D. in molecular identification of nematodes from the University of Leeds in England.