Cancer HotSpot Panel Key Features
- Target 54 Cancer Gene Hotspots
- Detect as low as 1% minor allele frequency
- Cover more than 13,000 mutations
- Start with as little as 250ng genomic DNA or FFPE derived DNA
A hotspot is a region of the genome containing known somatic mutations associated with cancer progression and resistance to therapy. The RainDance Cancer Hotspot Panel enables researchers to simultaneously interrogate hotspots across 54 genes and target more than 13,000 known mutations found in the Catalogue of Somatic Mutations in Cancer (COSMIC) database version 55, and discover new mutations in common tumor suppressor genes.
The panel leverages RainDance’s proprietary primer design pipeline to target the relevant regions and provide the highly accurate, consistent, and reproducible results required in cancer research.
Cancer Hotspot Panel Advantages
Sequence more somatic mutations
The most comprehensive cancer hotspot panel on the market. Simultaneously interrogate cancer hotspots across 54 genes and target more than 13,000 known somatic cancer mutations found in the COSMIC database.Detect rare and actionable mutations
Leverage RainDance’s DeepSeq™ Solution to detect mutations as low as 1%.Eliminate NGS library preparation
Reduces cost by eliminating library construction and size selection processes.Reduce sequencing cost
Increases on target reads to more than 90% with DirectSeq™ Workflow which enables greater sample multiplexing on next generation sequencers.Works with all Next Generation Sequencers
Unlike other cancer panels that only work with specific Next Generation Sequencers, you choose based on which sequencer you wish to use.
Comprehensive Content
The RainDance Cancer Hotspot Panel enables researchers to simultaneously interrogate more than 13,000 COSMIC mutations found on 54 genes using 660 amplicons (99kb) in a single assay.
Detecting Rare Somatic Mutations
To better understand the progression and prognosis of cancer, researchers need to be able to interrogate rare and actionable somatic mutations in heterogeneous samples.The RainDance Cancer HotSpot Panel enables researchers to detect known mutations and uncover new mutations that are represented in as little as 1% of a heterogeneous tumor. Based on the proven RainStorm™ single-molecule picodroplet PCR technology, the Cancer Hotspot Panel delivers high specificity, and accurate detection of rare somatic changes.
*The RainDance Cancer Hotspot Panel is for Research Use Only. Not for use in diagnostic procedures.
Related Content
Publication
Media Coverage
- Clinical Sequencing News: New Products: RainDance’s Cancer
- BizJournals: RainDance Releases Cancer Screening Product
Press Release
Webinar
Watch this webinar and learn how Dr. Olivier Harismendy from UC San Diego developed and implemented an assay that targets ~100kb of mutational hotspots in cancer. He dicussed its technical validity for the identification of low-prevalence mutations in heterogeneous cancer samples. Video Interview
Check out this interview with Olivier Harismendy at ASHG 2012 in San Francisco, CA. Discussed are the ideas, challenges and future direction of his translational laboratory in human genetics using Next Generation Sequencing and how RainDance has helped his laboratory.