The DirectSeq™ workflow eliminates several sample processing steps saving time on labor and lowers the total next generation sequencing operation cost by eliminating library construction and size selection processes.
Using one of RainDance’s fully automated instruments for target enrichment, the DirectSeq workflow applies a two-step Polymerase Chain Reaction (PCR) and then allows users to move straight to sequencing on any next generation sequencer. This is done by using RainDance’s tailed primers which incorporate Next Generation Sequencing (NGS) adaptors into the primer.
Eliminates hours of manual library construction and size selection processes
Automated processing: target enrichment followed by two rounds of PCR is completely walk-a-way
Eliminates $50-$100 per sample associated with library construction and size selection processes
Lowers sequencing costs by increasing sequencing specificity to >90% which creates more efficient use of the sequencer and allows for greater sample multiplexing
Customers design gene panels by providing RainDance with their target gene regions of interest and the next generation sequencer they intend to use. Here is the workflow before sequencing on a next generation sequencer:
RainDance Technologies® is making complex genetics simple. The company’s ultra-sensitive genomic tools enable research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-effective, and early detection of cancer and other inherited and infectious diseases. Major research institutions and laboratories around the world rely on the performance of RainDance systems. Based outside Boston, Massachusetts, the company supports customers using RainDrop® Digital PCR, as well as the ThunderStorm® and ThunderBolts™ Next-Generation Sequencing Enrichment Systems through its international sales and service operations, as well as a global network of distributors and commercial service providers. Both technology platforms have been recognized by The Scientist as Top 10 Innovations with the RainDrop System winning the award in 2014 and the ThunderStorm System winning the award in 2011.