Targeted Enrichment for Next-Gen Sequencing

The RainDance Targeted Sequencing System enables customers to apply Polymerase Chain Reaction (PCR), the gold-standard technique to target a specific region of the genome, more cost-effectively and at higher-throughput using any next-generation sequencer.

Complete Targeted Sequencing Solution

Automated Instrumentation Low, medium, and high-throughput instrumentation

Primer Panels Custom and defined content for genetic and cancer research

Reagents and Consumables Smart consumables manufactured by Sony DADC

Streamlined Workflow

RainDance’s target enrichment workflow is simple and will reduce hands-on time required to prepare samples for sequencing. It also reduces sequencing costs due to the high uniformity achieved with RainDance’s single-molecule, picodroplet for target enrichment.

Sample Primer Panels

PCR Droplets

Next-Gen Sequencing

Target Your Regions of Interest

• Single-molecule picodroplet PCR yields better sequence uniformity and more complete design coverage
• Each PCR droplet contains a uniform amount of genomic DNA, forward and reverse primers and PCR master mix
• Results are highly uniform single-plex PCR products
• Achieve greater than 99% design coverage for the target region of interest
• Target regions of the genome that cannot be targeted by hybridization methods

It Pays with Total Time and Total Cost per Sample Savings

Multiple factors can be attributed to the total cost per sample for target enrichment and next-generation sequencing. While target enrichment costs represent the upfront expense, the right method will impact the following factors to deliver significant time and cost savings in the downstream sequencing and data analysis.

• Primer Design

  Proper primer design eliminates off-target and excess sequencing time and costs

• Coverage

  More comprehensive coverage reduces additional Sanger sequencing validation

• Specificity

  High specificity maximizes on-target sequencing throughput and reduces downstream sequencing costs

• Uniformity

  Uniform sequence coverage optimizes coverage and reduces overall costs

• Data Analysis

  High on-target sequence reads reduce the amount of time needed to search against genomic databases