Last fall we announced a collaboration with Integrated DNA Technologies (IDT) to develop consumables specifically tailored to perform on our RainDrop™ Digital PCR System. This week, IDT published an article about some of the work we’ve been doing together in rare allele detection applications.
The focus of the article is around our collaborative work on an LNA-modified ZEN™ double-quenched probe. As part of IDT’s PrimeTime® qPCR product line, the ZEN™ double-quenched probes generate less background while increasing experiment end point signal, significantly boosting sensitivity and precision when compared to more traditional probes. The LNA modification increases the probe melting temperature (Tm) and thus provides even greater probe specificity over target regions as short as 14 nucleotides (nt).
Using this new LNA-modified ZEN probe design enables users of the RainDrop system to increase sensitivity and drop the lower limit of detection (LLOD) by as much as a factor of two. Using these probes on the RainDrop system has also been shown to improve positive calling, the degree with which probes are correctly binding to the desired target, and limit amplification of false positives.
This is an exciting example of how dPCR converts exponential, analog qPCR data to a linear, digital readout that makes possible highly precise analysis of the PCR product and opens up new opportunities for genetic analysis. Digital PCR’s capacity to obtain reliable and quantitative measurement of low frequency sequences will also produce real benefits for samples that contain rare alleles or biomarkers in a mixed cell population and for detecting low level pathogens.
If you are interested in learning more about the RainDrop Digital PCR system, please contact us by filling out this form. A RainDance representative will follow up with you to answer your questions and discuss how digital PCR might support your work.