HLASeq™ Research Screening Panel enables scientists to interrogate entire human leukocyte antigen region and major histocompatibility complex using next-generation sequencing technology
ORLANDO, Fla. – April 5, 2011 – RainDance Technologies, Inc., today announced the commercial availability of the HLASeq™ Research Screening Panel, a comprehensive genetic screening solution for human leukocyte antigen (HLA) and major histocompatibility complex (MHC) research using next-generation sequencing technology. The announcement was made at the annual American Association for Cancer Research (AACR) meeting in Orlando, Florida.
The HLA super locus is one of the most complex regions in the human genome due to the extreme levels of polymorphism and linkage disequilibrium. The region contains more than 200 protein-coding genes that play key roles in immune function and defense as well as 6,000 named alleles. Although many autoimmune diseases occur with low incidence, collectively they have high prevalence and affect millions of people around the world.
The systematic mapping of HLA associations for common diseases can only be done using next-generation DNA sequencing, but current cost and technology constraints have made it difficult for researchers to detect many of the important disease-specific susceptibility and protective markers. The RainDance HLASeq panel provides the most extensive coverage of genes in the MHC region, enabling comprehensive cataloging of variation in complex HLA-associated disorders without interference from closely related sequences across the genome.
“The ability to routinely interrogate the entire HLA region using next-generation sequencing has the potential to significantly advance our understanding of these essential players in the regulation of human immune function,” said Nicholas Schork, Ph.D., Adjunct Professor of Psychiatry, University of California, San Diego and Professor, The Scripps Research Institute. “We have been very impressed with the initial results we’ve generated using the RainDance HLASeq panel, particularly its coverage of the entire HLA super locus and the completeness of the data from all of the genes that encompass this complex region.”
The HLASeq Research Screening Panel is a single assay that enables scientists to interrogate the entire HLA super locus (a 3.8Mb gene-rich region on 6p21.3) with unprecedented and unbiased single-base resolution of both common and rare genomic mutations. The HLASeq panel tiles amplicons across the entire HLA super locus, providing scientists with the ability to apply next-generation sequencing technology to generate ultra-high resolution HLA typing.
The HLASeq panel was developed and validated in collaboration with Expression Analysis (EA), a long-time provider of services on the RDT 1000 platform, using RainDance’s proprietary primer design methods and microdroplet-based PCR.
“At EA, we continually seek out innovative solutions and collaborators with unique genomic know-how that help our customers push the boundaries of what’s possible in clinical research,” added Steve McPhail, President and CEO of EA. “We have been extremely impressed with the number of new applications and defined content panels being developed by RainDance, including the HLASeq panel which we will offer as part of our growing portfolio of genomic services.”
The RainDance HLASeq Research Screening Panel utilizes NGS technology to offer comprehensive coverage of the HLA super locus, including all Class I (HLA-A, -B, -C, -E, -F, -G, -K and -L) and Class II (HLA-DMA, -DOA, -DPA, -DQA, -DRA, -DMB, -DOB, -DPB1, -DQ1B1, -DQB2, as well as DRB1, -3, -4 and -5) genes, as well as several minor HLA antigen genes. HLASeq’s coverage spans all of the exons and introns for the genes found within this highly polymorphic region of genomic DNA.
“The HLASeq panel is the latest addition to a growing portfolio of RainDance’s research screening panels and provides customers with a powerful tool to discover and routinely analyze causative mutations associated with many of the most complex immune disorders,” said Roopom Banerjee, CEO of RainDance Technologies. “These new products are part of our commitment to deliver innovative solutions using our proprietary microdroplet technology, by enabling researchers to conduct studies that were impossible with existing technologies.”
The panel leverages RainDance’s innovative microdroplet-based platform, which produces more than one million picoliter-size droplets – each of which represents a single-molecule PCR reaction – in less than one hour. The power, simplicity and precision of the RainDance technology enable researchers to solve complex biological questions relating to organ transplantation, as well as autoimmune diseases and forensics, with unprecedented sensitivity and specificity.
For additional information about the new HLASeq Research Screening Panel please visit: www.RainDanceTech.com/products/HLASeq.
Additional Research Screening Panels
RainDance Technologies also offers the ASDSeq™ and XSeq™ Research Screening Panels, two comprehensive tools that enable researchers to simultaneously interrogate key genes known to be linked to X-chromosome disorders and syndromic forms of Autism Spectrum Disorder (ASD) using next-generation sequencing. For more information please visit: www.RainDanceTech.com.
2011 AACR Annual Meeting
RainDance will be showcasing its complete line of research screening panels as well as demonstrating its microdroplet-based platform in its exhibition booth (#2158). For more information about AACR, please visit www.aacr.org.
About RainDance Technologies
RainDance Technologies is pioneering the use of high-throughput microdroplet-based analysis in human health and life science research. The company’s core RainStorm™ technology generates millions of discrete droplets that can encapsulate a single molecule, cell or reaction and be digitally analyzed and sorted one at a time. The power, precision and simplicity of microdroplets enable researchers to answer complex questions with unprecedented sensitivity and quantitation. The complete RainDance solution includes automated instrumentation, customizable bioinformatics and high-value consumables and reagents for applications including targeted next-generation DNA sequencing, methylation, digital PCR and single cell analysis. Based in Lexington, Massachusetts, the company supports scientists around the world through its international sales and support operations and a global network of distributors and service providers. For more information, please visit www.RainDanceTech.com.
RainDance, RainStorm, ThunderStorm, Digital Biology are trademarks of RainDance Technologies, Inc. All other brands may be trademarks of their respective holders.
The RainDance ASDSeq, HLASeq, and XSeq Research Screening Panels are for Research Use Only – Not for use in diagnostic procedures.
1. Vega Genome Browser (release 42 – Mar 2011). Chromosome 6-COX: 1-1.
2. Robinson J, Waller MJ, Parham P, de Groot N, Bontrop R, Kennedy LJ, Stoehr P, Marsh SGE. IMGT/HLA and IMGT/MHC: sequence databases for the study of the major histocompatibility complex Nucleic Acids Research (2003), 31:311-314.