Date: Thursday, October 20, 2016
Title: The RainDrop dPCR system in a translational research environment: Methylation and Multiplexing on circulating tumour DNA.
Speaker: Fanny Garlan, M.Sc., Ph.D., Paris Descartes University (Paris, France)
Abstract: Circulating tumor DNA has previously been suggested as a biomarker for numerous cancer types. Picoliter-droplet digital PCR has also previously been proven as a powerful tool to precisely monitor ctDNA. Experiments coupling fluorescent probes to the RainDrop dPCR System permitted the detection of rare mutant clones using either multiplex or duplex assays screening up to 5 targets in a single experiment.
Recently, quantitative detection of methylated sequences emerged as an alternative marker to somatic mutations for ctDNA follow-up. Results on detection of ctDNA hyper-methylation and patients’ ctDNA monitoring will be presented together with an overview of technical considerations in dPCR use. CtDNA detection, through these recent developments, demonstrates appealing opportunities in the assessment of cancer samples.
Biography: As a generalist engineer, Fanny Garlan decided to use her scientific knowledge in a biotherapy-oriented field. She had experiences in neurobiology and also explored research exploitation in industrial property before starting her PhD in translational research. She participated to the development of digital PCR assays dedicated to biomarkers detection in liquid biopsies. Her work was focused on mutation and methylation detection in plasma, as well as the study of circulating tumor DNA integrity. In the course of her PhD, she participated to collaborations with both R&D scientists and physicians and co-authored two publications.