Prof. Dr. Dr. Torsten Haferlach of MLL Munich Leukemia Laboratory will soon present data on a new Chronic Lymphocytic Leukemia gene panel that was developed using the RainDance ThunderStorm™ Targeted DNA Sequencing platform.
Meeting: 11th Annual Symposium of the European LeukemiaNet
Date: February 4, 2014
Presentation Time: 17:30 CET
Location: Mannheim at the Congress Center Rosengarten
The team of scientists at MLL developed a targeted sequencing assay that is easily adoptable to adjust gene targets and amplicons according to current state-of-the-art evidence regarding the published landscape of mutations in CLL. In total, 13 genes with relevance in CLL were chosen: ATM, BIRC3, BRAF (V600), FBXW7, KLHL6, KRAS, NOTCH1 (PEST domain), NRAS, MYD88, POT1, SF3B1 (HEAT repeats), TP53, and XPO1. Targets of interest comprised either complete coding gene regions or hotspots. In summary, 323 amplicons were designed with a median length of 204 bp (range 150-240 bp), representing a total target sequence of 39.36 kb. The sequencing library was constructed starting off 2.2 μg genomic DNA per patient using the RainDance single-plex picrodroplet-based assay. Sequencing data was generated using the Illumina MiSeq instrument loading up to 10 patients per run. The total turn-around time of the assay was less than 5 days. As a proof-of-principle cohort, 18 clinically well-annotated CLL patients were analyzed during the evaluation phase. The median age was 78 years (range: 52 – 87 years).
The team will present data that demonstrate the ability to robustly target 13 genes for next-generation sequencing and the potential to screen for prognostically relevant mutations in all CLL patients in a fast and comprehensive way to ultimately, help guide therapy.
- Learn more about MLL and its service offering: http://www.mll.com/cms/englisch
- Read this article on MLL’s Myeloid Malignancy Panel, please visit: http://raindancetech.com/profile-story-prof-dr-torsten-haferlach-from-munich-leukemia/
- For more information on the European LeukemiaNet Symposium, please visit http://www.leukemia-net.org/content/home/calendar/index_eng.html?record_id=e10090
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