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Capable of generating more than a billion reactions in a single day, the RainDrop® Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation and multiplexing. Based on RainDance’s proven digital droplet platform, the RainDrop System fundamentally changes the performance of molecular assays by enabling digital answers across a number of important applications including low-frequency tumor allele detection, gene expression, copy number variation, and SNP measurement.
A new gold standard in sensitivity is only possible through the careful consideration of every aspect of the system. The RainDrop System offers a number of innovative digital droplet-based design advantages.
Don’t risk your research experiments to first-generation designs. The RainDrop System is the third generation of RainDance’s digital droplet-based PCR instrumentation and uses the fourth generation of our microfluidic chips. These innovations have a long track record of success in the lab and have been detailed by leading research institutions in publications including Analytical Chemistry, Lab on a Chip, and PNAS.
The RainDrop System shifts the PCR paradigm from a single color per marker approach to a more scalable and precise multi-color and intensity-per-marker method. This novel approach increases sensitivity by generating between 1 million and 10 million pico-liter sized droplets per lane, which is a 500 – 10,000x improvement over existing PCR methods. Since each droplet encapsulates a single molecule, researchers can quickly determine the absolute number of droplets containing specific target DNA and compare that to the amount of droplets with normal, background wild-type DNA.
To assess the lower limit of detection (LLOD) of an EGFR mutation, varying concentrations of mutant were diluted into genomic wild-type DNA. The assay delivered a linear response (R2 = 0.998) down to 1 mutant amongst 250,000 wild-type molecules, with a LLOD of 1 in more than 1,000,000 as defined by the average of the wild-type only controls plus 3x the standard deviation.
By adjusting concentrations of the probes for each individual assay, 4 mutations plus wild-type were measured simultaneously with just VIC and FAM fluorophores. Multiplexing can be expanded to higher plex levels, and it enables detection, identification, and measurement of multiple possible mutations from a single DNA sample.
The RainDrop Digital PCR System is for Research Use Only; not for use in diagnostic procedures.
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The RainDance team enjoyed meeting everyone who stopped by our booth at #ASHG14. Safe travels home!