The RainDance RainDrop System transforms the performance of molecular assays by enabling digital answers across a number of important applications including low-frequency tumor allele detection, gene expression, copy number variation, and SNP measurement.
Low-frequency allele detection
The RainDance RainStorm droplet-based microfluidics method utilizes up to 10 million reactions to enable detection of rare mutations in a background of wild-type DNA at levels better than 1 in 250,000. The combination of superior sensitivity, unprecedented multiplexing, and flexibility in experiment design provide a powerful genomic analysis platform for new research in cancer including rare variant detection, absolute quantitation of biomarkers, tumor profiling, and the ability to monitor residual disease.
Copy number variation
Copy number variations (CNVs) are involved in a large number of complex human diseases including many cancers and genetic conditions. An important measurement challenge in translational research involves identification of small CNV changes with high confidence. Cell free DNA (cfDNA) in blood plasma, for example, promises a readily available source of genetic material for tumor or pre-natal diagnosis with minimally invasive sampling techniques.
Early efforts using dPCR have reported the ability to detect a 1.25-fold difference in copy number 4. Additionally, the binary nature of dPCR means that the precision is more independent of variation in assay amplification, making it easier to optimize and standardize between laboratories. The RainDrop System is the only dPCR platform that is able to provide the required level of precision to measure smaller CNV differences in more challenging samples like cfDNA in a cost effective manner.
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